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Centre for Applied Biomedical Engineering Research Department of Mechanical, Aeronautical & Biomedical Engineering, Materials & Surface Science Institute, University of Limerick, Ireland

Cell Culture Facilities

CABER has established a well-equipped cell culture facility in order to support multiple experimental set-ups and tasks ranging from physiology, molecular biology, fluidics, biomaterial research and medical device development.

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Manipulation of the cell culture

Culture of cell lines and all experimental procedures related to mammalian cell culture are carried in Safety Cabinets. Experiment procedures include biocompatibility testing; viability and proliferation, functionality of biological material and constructs, and experiments dedicated to cellular physiology and pathophysiology. Supporting equipment such as a waterbath and microscopes allow for temperature controlled media and chemical components utilised in such studies as well as visual control of the cell densities and morphology during experimental manipulation.

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Cell propagation and physiological procedures (CO2 Incubator)

In order to facilitate the growth, proliferation, metabolism and function of cells in vitro, it is necessary to reproduce an environmental mimetic of the human or animal body in vivo. Numerous CO2 incubators are located in the CABER cell culture laboratory.

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Cryo-facilities - Cell preservation and long-term storage

CABER facilities also include cryopreservation of cryopreserved cell line stocks, allowing for long-time storage. Techniques for cell-line preservation which enable reliable and reproducible recovery of material with unchanged, defined characteristics are essential in many tissue engineering and cellular physiology studies.

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